Figure 6.

Overexpression of GFPT2 counteracts the protective roles of miR-27a in H/R-induced cell damage. A, GFPT2 expression in H/R-treated cells overexpressing miR-27a determined by western blot analysis 48 h after LV-GFPT2 administration (unpaired t test, ** p < 0.01 vs. miR-27a + LV-NC); B-C, viability of cells determined by EdU labeling (b) and CFSE labeling followed by flow cytometry (c) (unpaired t test, ** p < 0.01 vs. miR-27a + LV-NC); D-E, apoptosis of H9C2 cells determined by Hoechst 33342/PI double staining (d) and flow cytometry (e) (unpaired t test, ** p < 0.01 vs. miR-27a + LV-NC); F-G, mRNA (f) and protein (g) expression of Vimentin and α-SMA in cells determined by RT-qPCR and western blot analysis (two-way ANOVA, * p < 0.05, ** p < 0.01 vs. miR-27a + LV-NC); H, expression of TGF-β1 and phosphorylation of Smad2/3 in cells determined by western blot analysis (two-way ANOVA, * p < 0.05 vs. miR-27a + LV-NC). Data were exhibited as mean ± SD from at least three independent experiments