Skip to main content
. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Cell Metab. 2020 Dec 22;33(1):145–159.e6. doi: 10.1016/j.cmet.2020.12.005

Figure 4. Normal CD34+ cells express a low level of GLUT5, but their SSP flux is not higher in fructose-rich conditions compared to glucose-rich conditions.

Figure 4.

(A) Flow cytometry analysis of GLUT5 in MOLM13 and CD34+ cells (mean ± S.D., n = 3 biological replicates). MFI, median fluorescence intensity.

(B) Growth curves of CD34+ cells in 10 mM glucose or fructose media (mean ± S.D., n = 3 biological replicates).

(C) Changes in metabolites in the media from the experiment (B) (mean ± S.D., n = 3 biological replicates).

(D) Comparison of the intracellular level of serine and glycine and their enrichment in CD34+ cells cultured with 10 mM [U-13C] glucose or fructose for 2 days (mean ± S.D., n = 3 biological replicates).

All statistical analyses were conducted with unpaired two-tailed t-test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.