| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies for immunoblot | ||
| HK2 (C64G5), rabbit mAb (1:1000) | Cell Signaling Technology (CST) | # 2867 |
| Aldolase A (D73H4), rabbit mAb (1:1000) | CST | # 8060 |
| Aldolase B, rabbit pAb (1:1000) | Abcam | # ab153828 |
| LDHA, rabbit pAb (1:1000) | CST | # 2012 |
| Phospho-LDHA (Tyr10), rabbit pAb (1:1000) | CST | # 8176 |
| PHGDH, rabbit pAb (1:1000) | Sigma-Aldrich | # HPA021241 |
| Actin (D6A8) HRP-conjugated, rabbit mAb (1:10000) | CST | # 12620 |
| KHK, rabbit pAb (1:1000) | Abcam | # ab154405 |
| PKM1 (D30G6), rabbit mAb (1:1000) | CST | # 7067 |
| PKM2 (D78A4), rabbit mAb (1:1000) | CST | # 4053 |
| Phospho-PKM2 (Tyr105), rabbit pAb (1:1000) | CST | # 3827 |
| GCK, mouse mAb (1:1000) | R&D Systems | # MAB7840 |
| PKCζ (C24E6), rabbit mAb (1:1000) | CST | # 9368 |
| Phospho-PKCζ (190D10), rabbit mAb (1:1000) | CST | # 2060 |
| Glut5 (E-2), mouse mAb (1:1000) | Santa Cruz Biotechnology | # sc-271055 |
| Antibodies for flow cytometry | ||
| Human CD45 PerCP Cy5.5 clone 2D1 (1:100) | Thermo Fisher Scientific | # 45-9459-42 |
| Mouse CD45.1 V450 clone A20 (1:200) | Thermo Fisher Scientific | # 48-0453-80 |
| Mouse CD45 APC clone 30-F11 (1:200) | BD Biosciences | # 559864 |
| PHGDH, rabbit pAb (1:50) | Thermo Fisher Scientific | # PA5-24633 |
| Rabbit IgG (H+L) Alexa Fluor 488 (1:200) | Thermo Fisher Scientific | # A-11008 |
| Human Glut5 Alexa Fluor 647 (1:50) | R&D Systems | # FAB1349R |
| Biological Samples | ||
| Human acute myeloid leukemia specimens | MSK The Hematologic Oncology Tissue Bank (HOTB) | N/A |
| Commercial Assays | ||
| CD45 MicroBeads, human | Miltenyi Biotec | # 130-045-801 |
| NAD/NADH-Glo Assay kit | Promega | # G9071 |
| EnzyChrom Glucose Assay kit | BioAssay Systems | # EBGL-100 |
| EnzyChrom Fructose Assay kit | BioAssay Systems | # EFRU-100 |
| CellROX Deep Red Reagent | Thermo Fisher Scientific | # C10422 |
| Seahorse XF Cell Mito Stress Test Kit | Agilent | # 103015–100 |
| Chemicals, peptides, and recombinant proteins | ||
| Ketohexokinase (KHK) Inhibitor - Calbiochem | Sigma-Aldrich | # 420640 |
| NCT-503 | Cayman Chemical | # 19718 |
| CBR-5884 | Dr. Lewis C. Cantley group | # 19236 |
| DASA58 | Cayman Chemical | # 13941 |
| TEPP46 (ML-265) | Cayman Chemical | # 13942 |
| Puromycin dihydrochloride | Sigma-Aldrich | # P9620 |
| Doxycycline hyclate | Sigma-Aldrich | # D9891 |
| Cell-Tak Cell and Tissue Adhesive | Corning | # 354240 |
| N-acetyl-L-Cysteine | Sigma-Aldrich | # A7250 |
| Dimethyl alpha-ketoglutarate | Sigma-Aldrich | # 349631 |
| Recombinant Human SCF | PeproTech | # 300–07 |
| Recombinant Human IL6 | PeproTech | # 200–06 |
| Recombinant Human IL3 | PeproTech | # 200–03 |
| Recombinant Human Flt3-Ligand | PeproTech | # 300–19 |
| Recombinant Human GM-CSF | PeproTech | # 300–03 |
| Recombinant Human TPO | PeproTech | # 300–18 |
| 16% Paraformaldehyde (formaldehyde) aqueous solution | Electron Microscopy Sciences | # 15710-S |
| Annexin V-PE | BioLegend | # 640947 |
| DAPI | Thermo Fisher Scientific | # D1306 |
| Trypan Blue | Sigma-Aldrich | # T8154 |
| 2xLaemmli Buffer | Bio-Rad | #1610747 |
| ECL Western Blotting Substrate | Thermo Scientific | #1859698 #1859701 |
| 4–15% SDS-PAGE gel | Bio-Rad | #4561084 |
| Metabolites and enzymes | ||
| Phospho(enol)pyruvic acid monosodium salt hydrate (PEP) | Sigma-Aldrich | # P0564 |
| NADH (sodium salt hydrate) | Cayman Chemical | # 16078 |
| Adenosine 5′-diphosphate sodium salt (ADP) | Sigma-Aldrich | # A2754 |
| Sodium pyruvate | Thermo Fisher Scientific | # 11360070 |
| Sodium lactate | Sigma-Aldrich | # L7022 |
| [U-13C] Glucose | Sigma-Aldrich | # 389374 |
| [U-13C] Fructose | Sigma-Aldrich | # 587621 |
| [U-13C] Glutamine | Sigma-Aldrich | # 605166 |
| [2-13C] Glucose | Sigma-Aldrich | # 310794 |
| [2-13C] Fructose | Sigma-Aldrich | # 492140 |
| Thymidine | Sigma-Aldrich | # T1895 |
| Uridine | Sigma-Aldrich | # U3003 |
| Adenosine | Sigma-Aldrich | # A4036 |
| Cytidine | Sigma-Aldrich | # C4654 |
| Inosine | Sigma-Aldrich | # I4125 |
| Lactate dehydrogenase (LDHA) | Sigma-Aldrich | # SAE0049 |
| Pyruvate kinase (PK) | Sigma-Aldrich | # P9136 |
| Experimental Models: Cell Lines | ||
| KG1 | MSK | N/A |
| MOLM13 | MSK | N/A |
| TF1 | ATCC | # CRL-2003 |
| NOMO1 | MSK | N/A |
| MV411 | MSK | N/A |
| U937 | MSK | N/A |
| OCIAML2 | MSK | N/A |
| OCIAML3 | MSK | N/A |
| THP1 | MSK | N/A |
| KASUMI1 | MSK | N/A |
| K562 | MSK | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) | The Jackson Laboratory | #005557 |
| Software and Algorithms | ||
| Graphpad Prism | Graphpad | https://www.graphpad.com/ |
| Chenomx | Chenomx | https://www.chenomx.com |
| MestReNova | Mesterlab Research | https://mestrelab.com |
| FlowJo | BD Biosciences | https://www.flowjo.com/ |
| FACSDiva | BD Biosciences | https://www.bdbiosciences.com |
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