Fig. 3.

Essential role for hENT1 and dCK expression in the sensitivity of high-grade meningioma cells to gemcitabine. (A-F) HKBMM and/or IOMM-Lee cells were transfected twice with siControl, sihENT1 (#1 or #2), or sidCK (#1 or #2). (A and B) Transfected cells were harvested 3 days after the second transfection and subjected to Western blotting. (C and D) Alternatively, 2 days after the second transfection, transfected cells were cultured in the presence of gemcitabine for 3 days and then in the absence of gemcitabine for another 3 days and subjected to the WST-8 assay. (E and F) Transfected IOMM-Lee cells were seeded at a colony-forming density (500 cells/well in 6-well plates), cultured in the presence of gemcitabine for 3 days, and then in the absence of gemcitabine for another 6-9 days, to perform the colony formation assay. In (C) through (F), values were shown as the mean ± SD from samples in 6 replicates of a representative experiment repeated with similar results. *P < .05, NS: P ≥ .05. Abbreviations: dCK, deoxycytidine kinase; hENT1, human equilibrative nucleoside transporter 1; NS, nonsignificant.