Fig. 3.

Tumor-associated vasculature and tumor-derived endothelium are molecularly distinct. (A) Heatmap of differentially expressed genes detected by RNA-seq. Genes enriched are yellow, genes depleted are turquoise. Associated select gene ontology (GO) terms for genes up-regulated in either (B) bulk tumor or (C) TAV and TDEC populations. (D) Heatmap showing the individual genes for the GO categories Nervous System Development and Angiogenesis. (E) Gene Set Expression Analysis (GSEA) shows enrichment of VEGF-induced transcripts in the combined TAV and TDEC populations. (F) GSEA shows enrichment of CNS angiogenic genes in TAV and TDECs. (G) Heatmap of individual genes used for the angiogenic analysis (panel E) depicts their enrichment in the TAV and TDEC populations and depletion from bulk tumor.