Table 1.
Primer Sequences for RT-PCR
| Gene | Primer | Length |
|---|---|---|
| PARP | Upstream: 5′-TCGACGTGGAGAGCATGAAG-3′ Downstream: 5′-ATAGAGTAGGCGGCCTGGAT-3′ |
106bp |
| Caspase-3 | Upstream: 5′-GAGCTGGACTGCGGTATTGA-3′ Downstream: 5′-TAGTAACCGGGTGCGGTAGA-3′ |
113bp |
| Bcl-2 | Upstream: 5′-ACTCTTCAGGGATGGGGTGA-3′ Downstream: 5′-TGACATCTCCCTGTTGACGC-3’ |
94bp |
| Bax | Upstream: 5′- CATGTTT GCAGACGGCAACT-3′ Downstream: 5′-TGATCAGCTCGGGCACTTTA-3’ |
107bp |
| Actin | Upstream: 5′-GGAGAAGATTTGGCACCACAC-3′ Downstream: 5′-ACACAGCCTGGATGGCTACG-3’ |
173bp |