Fig 2. Comparison of lyophilized and evaporated Taq DNA polymerase cellular reagents prepared with or without solid support.

Activities of cellular reagents were compared by performing 20 cycle PCR amplification of 10 ng CT16S plasmid templates using either 3 μL of rehydrated evaporated or lyophilized cellular reagents prepared without solid support (‘Liquid’) or a single 3 mm paper disc containing evaporated cellular reagents added into the PCR reaction mix (PTR5 and GF800: two types of glass fiber conjugate pad paper; 691, 693, 161: three types of glass fiber filter paper). Agarose gel electrophoretic analysis of resulting PCR amplicons is depicted. Expected product size is indicated by an asterisk. Data shown are representative of eight biological replicates.