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. 2021 Jun 1;16(6):e0252507. doi: 10.1371/journal.pone.0252507

Fig 3. Activity of evaporated KlenTaq cellular reagents.

Fig 3

KlenTaq DNA polymerase cellular reagents evaporated at the indicated temperature with (GF800) or without (None) solid support were tested after 2 months of preparation by performing qPCR reactions with or without CT16S plasmid templates. Quantitative PCR reactions performed using commercially obtained pure KlenTaq enzyme served as control. Amplification curves observed in real-time by measuring increase in fluorescence of intercalating EvaGreen dye are depicted in panel A. Melting temperature analysis of resulting PCR amplicons is shown in panel B. Data shown are representative of three biological replicates.