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. 2021 Jun 1;16(6):e0252507. doi: 10.1371/journal.pone.0252507

Fig 4. Activity of Bst-LF evaporated cellular reagents.

Fig 4

Bst-LF DNA polymerase cellular reagents evaporated at the indicated temperature with (GF800) or without (None) solid support were tested after 2 months of preparation by performing LAMP-OSD reactions with or without human gapd plasmid DNA templates. LAMP-OSD reactions performed using commercially obtained pure Bst 2.0 enzyme or with lyophilized Bst-LF cellular reagents (Lyo CR) served as controls. Amplification curves observed in real-time by measuring increase in fluorescence of OSD probes are depicted. Data shown are representative of three biological replicates.