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. 2021 Jun 1;16(6):e0252507. doi: 10.1371/journal.pone.0252507

Fig 5. Activity and stability assays of evaporated OpenVent cellular reagents produced in Ghana and Cameroon.

Fig 5

(A) Left panel: OpenVent evaporated cellular reagents were used to amplify the synthetic beta-globin human gene. OpenVent is functional down to 25% of the standard concentration (2μL of reagent in 20μL PCR reaction). Right panel: OpenVent evaporated cellular reagents are stable for at least 7 months stored at 4°C in an airtight container in the presence of silica desiccant beads. (B) OpenVent evaporated cellular reagents amplify DNA fragments up to 7.5 kb from lambda genome. The shorter amplicons seen along with the 7.5 kb PCR product is due to unintended mispriming of lambda DNA. No amplicons were observed in the negative controls lacking lambda DNA templates. MW: molecular weight (A: 100bp DNA ladder, Newmarket Scientific, B: 1Kb plus DNA ladder, Beneficial Bio; C-: negative control).