Table 1.
Annotation of 10 Methylated Nucleosides and 6 Methylated Amino Acids for Workflow Validation
| No. | name | RT (min) | RI (Fiehn) | MINE ID | formula | mass | CFM-ID rank | MS-FINDER rank |
|---|---|---|---|---|---|---|---|---|
| 1 | 1-methyladenosine | 24.9 | 942720 | 602371 | C11H15N5O4-3TMS | 497.231 | 3 | 4 |
| 2 | 1-methylguanosine | 28.8 | 1102998 | 259583 | C11H15N5O5-4TMS | 585.265 | 8 | 1 |
| 3 | 1-methylpseudouridine | 23.1 | 872424 | 284529 | C10H14N2O6-3TMS | 474.204 | 2 | 2 |
| 4 | 2′-O-methylcytidine | 24.2 | 916966 | 602133 | C10H15N3O5-3TMS | 473.220 | 1 | 1 |
| 5 | 3′-O-methylcytidine | 23.9 | 905689 | 602134 | C10H15N3O5-3TMS | 473.220 | 2 | 2 |
| 6 | 3′-O-methylinosine | 23.1 | 870695 | 550471 | C11H14N4O5-3TMS | 498.215 | 2 | 2 |
| 7 | 3′-O-methyluridine | 22.0 | 828405 | 507523 | C10H14N2O6-3TMS | 474.204 | 2 | 1 |
| 8 | 5-methylcytidine | 24.2 | 914212 | 153249 | C10H15N3O5-4TMS | 545.259 | 2 | 5 |
| 9 | 5-methyluridine | 22.6 | 852669 | 521419 | C10H14N2O6-4TMS | 546.243 | 5 | 2 |
| 10 | N-methyladenosine | 23.8 | 901455 | 52386 | C11H15N5O4-3TMS | 497.231 | 1 | 2 |
| 11 | N-methylleucine | 10.8 | 384857 | 18421 | C7H15NO2-2TMS | 289.189 | 2 | 2 |
| 12 | N-methyllysine | 16.9 | 634380 | 23416 | C7H16N2O2-3TMS | 376.240 | 5 | 2 |
| 13 | N-methylphenylalanine | 15.4 | 570942 | 23901 | C10H13NO2-2TMS | 323.174 | 1 | 2 |
| 14 | N-methylserine | 12.0 | 430901 | 5876 | C4H9NO3-3TMS | 335.177 | 1 | 6 |
| 15 | N-methylthreonine | 12.5 | 451638 | 979 | C5H11NO3-3TMS | 349.192 | 1 | 6 |
| 16 | N-methyltyrosine | 18.8 | 704844 | 5520 | C10H13NO3-3TMS | 411.208 | 1 | 2 |