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Propagation of a high-angle ray in FiMic/FLFM (a) and SPOT (b). An example emission beam path from a fluorophore outside the field of view is shown. SP: sample plane; BFP: back focal plane of the objective lens; IIP: intermediate image plane; IP: image plane; OL: objective lens; A: objective aperture; MLA: microlens array; TL: tube lens; L1 and L2: lenses; FOV: field of view; AS: and aperture stop. The dash-dot line represents the optical axis of objective lens.
