Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 28;13:4271–4281. doi: 10.2147/CMAR.S297316

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Lin et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Overexpression of circRNA-TBC1D4 inhibited SH-SY5Y cell migration, but not proliferation and colony-formation. (A) SH-SY5Y cells were infected with lentivirus LV18-circTBC1D4 and LV18NC. qRT-PCR was conducted to detect circRNA-TBC1D4 levels. The bar graph showed that circRNA-TBC1D4 level of SH-SY5Y infected with LV18-circTBC1D4 was significantly higher than SH-SY5Y infected with LV18NC. (B) CCK-8 assay of SH-SY5Y with circRNA-TBC1D4 overexpression and NC on the indicated days. The growth curve showed that overexpression of circRNA-TBC1D4 did not inhibit the proliferation of SH-SY5Y cells. There is no significant difference between SH-SY5Y with circRNA-TBC1D4 overexpression and NC. (C) Colony formation assays of SH-SY5Y with circRNA-TBC1D4 overexpression and NC. The left panel visually showed that overexpression of circNRA-TBC1D4 did not inhibit the colony-forming ability of SH-SY5Y cells. And the bar graph showed that there was no significant difference between SH-SY5Y with circRNA-TBC1D4 overexpression and NC. (D) Transwell migration assay of SH-SY5Y with circRNA-TBC1D4 overexpression and NC. The left panel visually showed that overexpression of circNRA-TBC1D4 significantly inhibited the migration ability of SH-SY5Y cells. The bar graph showed that there was significant difference between SH-SY5Y with circRNA-TBC1D4 overexpression and NC. Data are shown as means ± s.d. of at least three independent experiments. ****P < 0.0001; ns, P > 0.05.