KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-GFP | Novus Biologicals | Cat#NB600–308; RRID:AB_10003058 |
| Bacterial and Virus Strains | ||
| AAV-DJ hSyn-FLEX-mGFP-2A-Synaptophysin-mRuby | Stanford Virus Core | Cat#GVVC-AAV-100 |
| R(G)-lentivirus-hSyn-Cre | Wang Lab, MIT | Addgene plasmid #86641 |
| AAV2/2-EF1α-DIO-EYFP | UNC Vector Core | N/A |
| R(G)-EIAV-DIO-FLP | Lim Lab, UCSD | N/A |
| AAV-DJ-EF1α-fDIO-EYFP | UNC Vector Core | N/A |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| NeuroTrace™ 435/455 Blue Fluorescent Nissl Stain | ThermoFisher | Cat#N21479 |
| Alexa 594-conjugated dextran | ThermoFisher | Cat#D22913 |
| Kynurenic acid sodium salt | Abcam | Cat#ab120256 |
| Picrotoxin | Abcam | Cat#ab120315 |
| OPAL 520 | Akoya Biosciences | Cat#FP1487001KT |
| OPAL 620 | Akoya Biosciences | Cat#FP1495001KT |
| OPAL 690 | Akoya Biosciences | Cat#FP1497001KT |
| Critical Commercial Assays | ||
| RNAscope® Multiplex Fluorescent Reagent Kit v2 | ACD | Cat#323100 |
| Vectastain® Elite ABC-HRP Kit | Vector | Cat#PK-6100; RRID:AB_2336819 |
| RNAscope® Probe -Mm-Pvalb | ACD | Cat#421931 |
| RNAscope® Probe -Mm-Slc32a1-C2 | ACD | Cat#319191-C2 |
| RNAscope® Probe -Mm-Th-C3 | ACD | Cat#317621-C3 |
| RNAscope® Probe -Mm-Gad2-C3 | ACD | Cat#439371-C3 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| B6.129P2-Pvalbtm1(cre)Arbr/J | The Jackson Laboratory | Stock No. 017320; RRID:IMSR_JAX:017320 |
| C57BL/6J | The Jackson Laboratory | Stock No: 000664; RRID:IMSR_JAX:000664 |
| B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/MwarJ | The Jackson Laboratory | Stock No: 028862; RRID:IMSR_JAX:028862 |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| ImageJ | NIH |
https://imagej.nih.gov; RRID:SCR_003070 |
| MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html; RRID:SCR_001622 |
| Python, algorithms | Chen et al., 2019 | https://github.com/mistycheney/MouseBrainAtlas; RRID:SCR_008394 |
| VTK Visualization Toolkit |
http://vtk.org; RRID:SCR_015013 |
|
| Neurolucida | MBF Bioscience | https://www.mbfbioscience.com/neurolucida; RRID:SCR_001775 |
| Igor Pro 6 | Wavemetrics | https://www.wavemetrics.com/order/order_igordownloads6.htm; RRID:SCR_000325 |
| ZEN Digital Imaging Blue 2.0 | Carl Zeiss Microscopy GmbH | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html; RRID:SCR_013672 |
| QuPath v0.2.1 | Bankhead et al., 2017 |
https://qupath.github.io; RRID:SCR_018257 |
| Other | ||
| Axio Scan Z.1 Slide scanner microscope | Carl Zeiss Microscopy GmbH | N/A |
| ORCA-4.0 V3 digital CMOS camera | Hamamatsu | Cat# C13440–20CU |
| Sliding Microtome | Microm International | Cat #HM400 |
| Nanoject II Auto-Nanoliter Injector | Drummond Scientific | Cat#3–000-204 |
| Microinjector | Creative Instruments Development Company | N/A |
| Multiclamp 700B Microelectrode Amplifier | Molecular Devices | RRID:SCR_018455 |
| ITC-18 | HEKA | N/A |
| P-97 Flaming/Brown Micropipette Puller | Sutter | RRID:SCR_018636 |
| DMZ Universal Electrode Puller | Zeitz-Instruments | RRID:SCR_014774 |
| Vibratome VT1200S | Leica Biosystems | RRID:SCR_018453 |