| Genetic reagent (D. melanogaster) |
w1118
|
Bloomington Drosophila Stock Center |
BDSC:3605; FLYB: FBst000360; RRID:BDSC_3605
|
w[1,118] |
| Genetic reagent (D. melanogaster) |
w1118; P{UAS-NLS-NES+-GFP}5A |
Bloomington Drosophila Stock Center |
BDSC:7032; FLYB: FBst0007032; RRID:BDSC_7032
|
w[1,118]; P{w[+ mC] = UAS-NLS-NES[+]-GFP}5A |
| Genetic reagent (D. melanogaster) |
y1 w*; P{UAS-NLS-NESP12-GFP}2A |
Bloomington Drosophila Stock Center |
BDSC:7033; FLYB: FBst0007033; RRID:BDSC_7033
|
y (Amador-Ortiz et al., 2007) w[*]; P{w[+ mC] = UAS-NLS-NES[P12]-GFP}2A |
| Genetic reagent (D. melanogaster) |
w1118; P{EP}Nup44AEP2417
|
Bloomington Drosophila Stock Center |
BDSC:17053; FLYB: FBst0017053; RRID:BDSC_17053
|
w[1,118]; P{w[+ mC] = EP}Nup44A[EP2417] |
| Genetic reagent (D. melanogaster) |
w1118; P{EPg}Nup93-2HP35056
|
Bloomington Drosophila Stock Center |
BDSC:21975; FLYB: FBst0021975; RRID:BDSC_21975
|
w[1,118]; P{w[+ mC] = EPg}Nup93-2[HP35056] |
| Genetic reagent (D. melanogaster) |
P{VSH330104}attP40 |
Vienna Drosophila Resource Center |
VDRC:v330104; FLYB: FBst0491076; RRID:FlyBase_FBst0491076
|
P{VSH330104}attP40 |
| Genetic reagent (D. melanogaster) |
P{KK108318}VIE-260B |
Vienna Drosophila Resource Center |
VDRC: v100588; FLYB: FBst0472461; RRID:FlyBase_FBst0472461
|
P{KK108318}VIE-260B |
| Genetic reagent (D. melanogaster) |
UAS-Nup62 |
This paper |
|
Created at BestGene |
| Genetic reagent (D. melanogaster) |
M{UAS-Nup214.ORF}ZH-86Fb |
FLYORF |
FLYORF: F001467; FLYB: FBst0500182; RRID:FlyBase_FBst0500182
|
M{UAS-Nup214.ORF}ZH-86Fb |
| Genetic reagent (D. melanogaster) |
M{UAS-Nup43.ORF.3xHA.GW}ZH-86Fb |
FLYORF |
FLYORF: F003133; FLYB: FBst0502466; RRID:FlyBase_FBst050246
|
M{UAS-Nup43.ORF.3xHA.GW}ZH-86Fb |
| Genetic reagent (D. melanogaster) |
CRISPR/Cas9 hTDP43-WT |
Gift from David B. Morton Chang and Morton, 2017
|
|
|
| Cell line (Homo sapiens) |
Human Embryonic Kidney cells (HEK 293T) |
ATCC |
RRID:CVCL_0063
|
|
| Transfected construct (human) |
mRFP-FKBP1A Plasmid |
Addgene |
(Cat #: 67514); RRID:Addgene_67514
|
transfected construct (human) |
| Transfected construct (human) |
Frt-V5-HspB2 Plasmid |
Addgene |
(Cat #: 63103); RRID:Addgene_63103
|
transfected construct (human) |
| Transfected construct (human) |
FLAG NM23-H1/NME1 Plasmid |
Addgene |
(Cat #: 25000); RRID:Addgene_25000
|
transfected construct (human) |
| Transfected construct (human) |
pEGFP SF2/SRSF1 Plasmid |
Addgene |
(Cat #: 17990); RRID:Addgene_17990
|
transfected construct (human) |
| Transfected construct (human) |
Nup54-HA-eGFP Plasmid |
VectorBuilder |
This paper |
transfected construct (human) |
| Transfected construct (human) |
HA-eGFP Plasmid |
VectorBuilder |
This paper |
transfected construct (human) |
| Transfected construct (human) |
mRuby Plasmid |
Gift from Dr. Christopher Donnelly |
This paper |
transfected construct (human) |
| Transfected construct (human) |
NUP62-mRuby Plasmid |
Gift from Dr. Christopher Donnelly |
This paper |
transfected construct (human) |
| Antibody |
anti-Nup214 (Guinea Pig polyclonal) |
Gift from Dr. Christos Samakovlis Roth et al., 2003
|
|
WB (1:5000) |
| Antibody |
Anti-Lamin Dm0 (Mouse monoclonal) |
DSHB |
Cat# ADL84.12; RRID:AB_528338
|
IF(1:200)WB (1:1000) |
| Antibody |
anti-α-Tubulin (Mouse monoclonal) |
Sigma-Aldrich |
Cat#: T5168; RRID:AB_477579
|
WB (1:10,000) |
| Antibody |
anti-Tbph (Rabbit polyclonal) |
Gift from Dr. Frank Hirth Diaper et al., 2013
|
|
IF (1:1500)WB (1:3000) |
| Antibody |
anti-FLAG (Mouse monoclonal) |
Sigma-Aldrich |
Cat#: F1804; RRID:AB_259529
|
IF (1:1000) |
| Antibody |
anti-GFP (Chicken polyclonal) |
Abcam |
Cat#: ab13970; RRID:AB_300798
|
IF (1:1000) |
| Antibody |
anti-TDP43 (Rabbit polyclonal) |
Proteintech |
Cat#: 10782–2-AP; RRID:AB_615042
|
IF (1:1000) |
| Antibody |
anti-phospho-TDP43 (Rat monoclonal) |
Millipore SIGMA |
Cat#: MABN14; RRID:AB_11212279
|
IF (1:1000) |
| Antibody |
anti-Mab414 (Mouse monoclonal) |
Abcam |
Cat#: ab24609; RRID:AB_448181
|
IF (1:1000) |
| Antibody |
anti-RanGAP1 (Rabbit polyclonal) |
Millipore SIGMA |
Cat#: ABN1674 |
IF (1:200) |
| Antibody |
anti-Nup62 (Mouse polyclonal) |
BD Transduction Laboratories |
Cat#: 610497; RRID:AB_397863
|
IHC/IF (1:500) |
| Antibody |
anti-pTDP-43 (Rabbit polyclonal) |
Cosmo Bio |
Cat#:NC0877946; RRID:AB_1961899
|
IF (1:1000) |
| Antibody |
anti-Nup62 (Mouse polyclonal) |
Roche Applied Science |
Cat#: 610497; RRID:AB_397863
|
IHC (1:400) |
| Antibody |
anti-RanGAP1 (Rabbit polyclonal) |
Santa Cruz Biotechnology |
Cat#: sc-28322; RRID:AB_2176987
|
IHC (1:1000) |
| Sequence-based reagent |
Futsch
|
This paper |
PCR primers (5'–3'): |
Forward: CAAAGCCCACTCACCTTTCReverse: CTGCTCCTGCCAACATCTProbe: AGTCTCTGGAAATGCAGCACCACT |
| Sequenced-based reagent |
dNup93-2
|
This paper |
PCR primers (5'–3'): |
Forward: ACACCGTCCGCGAAATACReverse: ACTCAACCGCCACCTTAACProbe: ATGGCCGCTGGTTTACTACGGATT |
| Sequenced-based reagent |
dNup214
|
This paper |
PCR primers (5'–3'): |
Forward: CCTAAGTGAGGACAAGGATGAGReverse: GGCATAGTCTGCAGCTTCTTProbe: TGCCTTCGACACTTCTACAACGCA |
| Sequenced-based reagent |
dNup54
|
This paper |
PCR primers (5'–3'): |
Forward: GAGTGAGCTGACAGAACTCAAGReverse: CTCGGCCAGTTTCCGTTTATProbe: CCACTGCCACAGCGAAGATACTTGA |
| Sequenced-based reagent |
dNup44A
|
This paper |
PCR primers (5'–3'): |
Forward: AAGGTATCCTCCACCAATACCCReverse: TTGGGTGCAAACTCCACATCProbe: TTGTAGACTCGCGGACCAGTGTCA |
| Sequenced-based reagent |
dNup62
|
This paper |
PCR primers (5'–3'): |
Forward: CTTGCTGTTGTCTGCATCTCReverse: CAGCACCAGCTTCAGGAProbe: ACATTCTCTTTCGGAACACCGGCA |
| Sequenced-based reagent |
Emb (Exportin)
|
This paper |
PCR primers (5'–3'): |
Forward: GGTCACGCGTATGTCATTCAReverse: GTTCACATTGACGCCATTCACProbe: AGCATGTCCAGATATATGCGGCCC |
| Sequenced-based reagent |
dNup43
|
This paper |
PCR primers (5'–3'): |
Forward: TTGCATCTGATCCTCCTCCACReverse: ACCGCCATGGAGTTCGTProbe: TGGACGTTAAACACGCTGAGATGACC |
| Sequenced-based reagent |
dGapdh
|
This paper |
PCR primers (5'–3'): |
Forward: CAACAGTGATTCCCGACCAGReverse: TTCGTCAAGCTAATCTCGTGGProbe: CCAAAACTATCGTACAAACCCGGCG |
| Commercial assay or kit |
3,3'-diaminobenzidine (DAB) |
Vector Laboratories |
Cat: #SK-4100; RRID:AB_2336382
|
|
| Commercial assay or kit |
NE-PER nuclear-cytoplasmic extraction kit |
ThermoFisher Scientific |
Cat #: 78,833 |
|
| Commercial assay or kit |
Nup62 ELISA |
LSBio |
Cat #: LS-F22196
|
|
| Chemical compound, drug |
KPT-350 |
Karyopharm |
|
|
| Chemical compound, drug |
KPT-276 |
Selleck Chem |
Cat #: S7251 |
|
| Software, algorithm |
GraphPad Prism 6 |
GraphPad Prism 6 |
RRID:SCR_002798
|
|
