Figure 3. Variability is weakly coupled to modal germination time.

(A) Scatter plots of coefficient of variation (CV) of germination time versus mode days to germination for 341 MAGIC lines. Each point is a specific MAGIC line, and in the majority of cases, the CV and mode are mean values obtained from sowing one batch of seeds from each of three separate parent plants. Each point is shaded according to the percentage germination of the line (see scale bar). Coloured circles and labels indicate lines for which examples are shown in (B–E). (ii) is a zoom in of (i) including only lines with CV < 0.6. Spearman’s correlation for the full set of 341 MAGIC lines is indicated in (i). (B–E) Distributions of germination times for pairs of MAGIC lines. The colour of the box matches the coloured circles in (A). Lower CV lines are shown on top. Grey-coloured bars show the germination time distribution of seed batches from replicate mother plants. (B, C) Exemplar lines with the same mode days to germination but different CVs of germination time. (D, E) Lines that have different CVs and different mode days to germination. For each line, the mode days to germination, final percentage germination and CV of germination time are shown. Note that the x-axis scale differs between plots. Figure 3—figure supplement 1 shows the relationship between CV and percentage germination for MAGIC lines. Figure 3—figure supplement 2 shows relationships between CV, mode and percentage germination for natural accessions. Figure 3—source data 1 contains source data for (A). Figure 3—source data 2 contains source data for (B–E).

Figure 3—figure supplement 1. Variability is weakly coupled to percentage germination.

(A) Scatter plots of coefficient of variation (CV) of germination time versus percentage germination for 341 MAGIC lines. Each point is a specific MAGIC line, and in the majority of cases, the CV and percentage are mean values for three batches of seeds from separate parent plants. Coloured circles and labels indicate lines for which examples are shown in (B, C). (ii) is a zoom in of (i), including only lines with CV < 0.6. Spearman’s correlation for the full set of 341 MAGIC lines is indicated in (i). (B, C) Distributions of germination times for exemplar MAGIC lines that have similar final percentage germination and mode days to germination but different CVs. The colour of the box matches the lines to the coloured circles in (A). Lower CV lines are shown on top. Grey-coloured bars show the distribution of germination time for seed batches from replicate mother plants. Note that the x-axis scale differs between (B) and (C). Figure 3—source data 1 contains source data for (A). Figure 3—source data 2 contains source data for (B) and (C).

Figure 3—figure supplement 2. The relationship between coefficient of variation (CV), mode days to germination and percentage germination in natural accessions.

(A) Scatter plot of CV of germination time versus mode days to germination for the 19 parental accessions of the MAGIC lines and 10 Spanish accessions. Each point is a specific accession, and the CV and mode are means for one batch of seed from each of at least three separate parent plants. Each point is shaded according to the percentage germination of the line (see scale bar). Orange circles indicate lines for which examples are shown in (C). Spearman’s correlation for the 29 accessions is indicated. (B) As for (A), but showing CV versus percentage germination. (C) Distribution of germination times for exemplar accessions, with similar mode and percentage germination but different CVs (accessions indicated in A). Grey-coloured bars show the distributions of germination for seed batches from replicate mother plants. Figure 3—figure supplement 2—source data 1 contains the source data for (A) and (B). Figure 3—figure supplement 2—source data 2 contains the source data for (C).