Figure 5. Kinematic rhythms synchronize with the sniff cycle selectively during olfactory search.

(i–iii) Nose speed, yaw velocity, and Z-velocity, respectively (see Figure 4 for definitions). (A) Top: color plot showing movement parameter aligned to inhalation onset for within-trial sniffs taken before crossing the decision line. Taken from one mouse, one behavioral session. Dotted line at time 0 shows inhalation onset, the second line demarcates the end of the sniff cycle, sorted by duration. Data are taken from one behavioral session. Middle: color plot showing each movement parameter aligned to inhalation onset for inter-trial interval sniffs taken before the first attempt at premature trial initiation. Bottom: sniff-aligned average of each movement parameter. Thin lines represent individual mice (n = 11), bolded lines and shaded regions represent the grand mean ± standard deviation. Green: within-trial sniffs; pink: inter-trial interval sniffs. (B) Normalized cross-correlation between movement parameter and sniff signal for the same sniffs as above. (C) Spectral coherence of movement parameter and sniff signal for the same sniffs as above.

Figure 5—figure supplement 1. Sniff synchronization shuffle test.

Sniff-aligned grand mean (n = 11 mice) of (A) nose speed, (B) yaw velocity, and (C) Z-velocity for within-trial (top) and inter-trial interval (bottom) sniffs, overlaid on 1000 iterations of trial-shuffled grand means. Thin black lines represent individual iterations, all of which are shown.

Figure 5—figure supplement 2. Kinematic rhythms for premature initiations during the inter-trial interval and between decision line and reward port during trials.

Sniff-aligned average of (A) nose speed, (B) yaw velocity, and (C) Z-velocity. Thin lines represent individual mice (n = 11), bolded lines and shaded regions represent the grand mean ± standard deviation. Top: green: within-trial sniffs from the time between crossing the decision line and entering the reward port. Pink: inter-trial interval sniffs from the time between the first premature trial initiation attempt and the successful initiation of the next trial. Bottom: green: within-trial sniffs at nose speeds above the threshold 15 cm/s. Pink: inter-trial interval sniffs at nose speeds above the threshold 15 cm/s.