Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | A549 | ATCC | CCL-185, RRID:CVCL_0023 | |
| Cell line (Homo sapiens) | NCI-H1299 | ATCC | CRL-5803, RRID:CVCL_0060 | |
| Cell line (Homo sapiens) | NCI-H1573 | ATCC | CRL-5877, RRID:CVCL_1478 | |
| Transfected construct (human) | mVenus-hGeminin (1/110) (plasmid) | Sakaue-Sawano et al., 2008 | ||
| Transfected construct (human) | mCherry-hCdt1 (30/120) (plasmid) | Sakaue-Sawano et al., 2008 | ||
| Transfected construct (human) | Cell Cycle-Chromobody plasmid | Chromotek | ccr | Recloned into a pLVX lentiviral backbone with TagRFP replaced with mNeonGreen |
| Transfected construct (human) | 53BP1trunc-Apple | Addgene | 69531, RRID:Addgene_69531 | 53BP1 C-terminally fused to mApple fluorescent protein Yang et al., 2015 |
| Transfected construct (human) | B_mCherry_IRES_neo3 | Addgene | 21044, RRID:Addgene_21044 | Steigemann et al., 2009 |
| Transfected construct (human) | LeGO-Cer2 (Cerulean) | Addgene | 27338, RRID:Addgene_27338 | Weber et al., 2008 |
| Transfected construct (human) | LeGO-V2 (Venus fluorescent protein) | Addgene | 27340, RRID:Addgene_27340 | Weber et al., 2008 |
| Transfected construct (human) | LeGO-C2 (mCherry) | Addgene | 27339, RRID:Addgene_27339 | Weber et al., 2008 |
| Antibody | Anti-p21 Waf1/Cip1 (Rabbit monoclonal) | Cell Signaling Technology | 2947, RRID:AB_330945 | Flow (1:200) |
| Antibody | Anti-p16 Ink4a (Mouse monoclonal) |
Abcam | AB201980, RRID:AB_2891086 | Flow (1:200) |
| Antibody | Anti-gamma H2A.X (phospho S139) antibody (Rabbit monoclonal) |
Cell Signaling Technology | 9718, RRID:AB_2118009 | Flow (1:200) |
| Antibody | Anti-PCNA (Mouse monoclonal) |
Abcam | AB29, RRID:AB_303394 | IHC (1:500−1:2000) |
| Antibody | Alexa Fluor Plus 647 Secondary Antibody (Rabbit polyclonal) |
Thermo Fisher Scientific | RRID:AB_A32733, RRID:AB_2633282 | Flow (1:500) |
| Antibody | BrdU-FITC (Mouse monoclonal) |
BD Biosciences | 347583, RRID:AB_400327 | Flow (1:20) |
| Chemical compound, drug | Alexa Fluor 647 Phalloidin | Thermo Fisher Scientific | RRID:AB_A22287, RRID:AB_2620155 | IF (1:40,000) |
| Chemical compound, drug | Cisplatin | Hospira Australia | 88S035 | |
| Chemical compound, drug | Carboplatin | Abcam | ab120828 | |
| Chemical compound, drug | Olaparib | Selleck Chem | S1060 | |
| Chemical compound, drug | Palbociclib | Selleck Chem | S1116 | |
| Chemical compound, drug | RI-1 | Selleck Chem | S8077 | |
| Chemical compound, drug | PureLink RNase A | Thermo Fisher Scientific | 12091021 | |
| Chemical compound, drug | C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside) | Thermo Fisher Scientific | D2893 | |
| Chemical compound, drug | Η33342 | Sigma | B2261 | 1 µg/ml |
| Chemical compound, drug | Propidium Iodide | Thermo Fisher Scientific | P3566 | |
| Chemical compound, drug | Thymidine | Selleck Chem | S4803 | |
| Chemical compound, drug | BrdU (5-Bromo-2′-Deoxyuridine) | Thermo Fisher Scientific | B23151 | |
| Commercial assay or kit | ImaGene Green C12FDG lacZ Gene Expression Kit | Molecular Probes | I2904 | |
| Other | Matrigel Basement Membrane | Bio-Strategy | BDAA354230 | |
| Software | Fiji/Image J | NIH | RRID:SCR_002285 | https://imagej.net/Fiji (Schindelin et al., 2012) |
| Software, algorithm | FlowJo | BD Biosciences | RRID:SCR_008520 | https://www.flowjo.com |
| Software, algorithm | GraphPad Prism (v9.1.0) | GraphPad | RRID:SCR_002798 | https://www.graphpad.com |
| Software, algorithm | Huygens Professional | Scientific Volume Imaging (SVI) | RRID:SCR_014237 | https://svi.nl/Huygens-Professional |
| Software, algorithm | LAS-X | Leica | RRID:SCR_013673 | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |