Table 1.

The pulling force averaged over every 1-nm interval with Nrp1 bound and without Nrp1

d (nm)	Force (pN) w Nrp1	Force (pN) w/o Nrp1	Δ (pN)	p
0–1	935	833	102	∗∗∗
1–2	881	814	66	∗∗∗
2–3	614	500	113	∗∗∗
3–4	511	380	131	∗∗∗
4–5	441	377	63	∗∗∗
5–6	393	341	51	∗∗∗
6–7	384	383	0	0.98
7–8	434	460	−26	∗
8–9	443	512	−69	∗∗∗
9–10	518	663	−144	∗∗∗
10–11	433	680	−246	∗∗∗
11–12	412	582	−169	∗∗∗
12–13	392	431	−39	∗
13–14	331	357	−26	0.03
14–15	239	313	−73	∗∗∗
15–16	218	270	−52	∗∗∗
16–17	206	235	−28	0.03
17–18	245	236	9	0.41
18–19	246	242	3	0.77
19–20	236	251	−15	0.22

The difference and significance (p-values) are calculated over simulation trajectories every 1 ns interval (saved at every 100 fs or 100 steps)

^∗p < 0.01;

^∗∗∗p < 0.0001; w, with; w/o, without.