Figure 4.

Sirt3 deficiency abolished the renoprotection of melatonin by deteriorating renal function and increasing renal tubular injure score in vivo. (a) The ratio of KW to BW in four groups. (b, c) The level of SCr and BUN in four groups. (d, e) Representative immunohistochemical staining and quantitative analysis of NGAL. (f) Representative micrographs of HE-stained kidney on morphological changes. (g–j) Semiquantitative analysis of interstitial edema, cytoplasmic vacuolar changes, intratubular cast formation, and luminal congestion to evaluated renal tubular injures. All experiments were performed at least 3 times. Data are expressed as mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.001.