Figure 5.

Sirt3 deficiency abolished the antioxidative effects of melatonin following iohexol. (a) Representative western blots. (b) Sirt3 expression. (c) ac-SOD2 K68 expression. (d) Nox4 expression. (e, f) Representative immunohistochemical staining and quantitative analysis of Nox4. (g) Renal MDA content. (h) Renal SOD activity. (i) Renal GSH-Px activity. (j–l) Quantitative analysis of HO-1, Nrf2, and Catalase mRNA expression in the kidney of different groups. (m–o) Quantitative analysis of IL-1β, TNFα, and TGFβ mRNA expression in the kidney of different groups. All experiments were performed at least 3 times. Data are presented as mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.001.