Figure 7.

Effect of melatonin administration on oxidative stress, apoptosis, and inflammation in iohexol-injured NRK-52E cells in vitro. (a) Representative western blots. (b–f) Quantitative analysis of Sirt3, ac-SOD2 K68, Nox4, Bax/Bcl2, and cleaved-caspase3 expression. (g) ROS production. (h–j) Quantitative analysis of HO-1, Nrf2, and Catalase mRNA expression in NRK-52E cells of four groups. (k–m) Quantitative analysis of TNFα, IL-1β, and TGFβ mRNA expression in NRK-52E cells of four groups. All experiments were repeated at least 3 times. Data are expressed as mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.001.