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. 2021 May 25;2021:6668887. doi: 10.1155/2021/6668887

Figure 8.

Figure 8

Sirt3 siRNA abolished the antioxidative, antiapoptotic, and anti-inflammatory effects of melatonin in iohexol-injured NRK-52E cells. (a–c) The knockdown efficacy of Sirt3 siRNA in NRK-52E cells. (d) Representative western blots. (e–i) Quantitative analysis of Sirt3, ac-SOD2 K68, Nox4, Bax/Bcl2, and cleaved-caspase3 expression. (j) ROS production. (k–m) Quantitative analysis of HO-1, Nrf2, and Catalase mRNA expression in NRK-52E cells of four groups. (n–p) Quantitative analysis of TNFα, IL-1β, and TGFβ mRNA expression in NRK-52E cells of four groups. All experiments were calculated at least 3 times. Data are expressed as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.001.