The impact of UCMS and UCMS+AET on EDD with and without tPVAT was tested by methacholine-induced relaxation of thoracic aortic rings using wire myograph in LZR (A&B) and OZR (C&D) groups. n=6–8 in each group or treatment. *denotes significant differences within treatments i.e., aortic ring with tPVAT relaxation compared to aortic ring with tPVAT relaxation or aortic ring only compared to aortic ring only. #denote significant effect of tPVAT, i.e., comparing aortic ring only to aortic ring with tPVAT. *p<0.05, **p<0.01, *** p<0.001, **** p<0.0001, #p<0.05, ##p<0.01 as measured by two-way repeated measures ANOVA with holm-sidak post-hoc analysis.