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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Exp Physiol. 2021 May 14;106(6):1343–1358. doi: 10.1113/EP089449

Figure 3. Unpredictable chronic mild stress (UCMS) impairment of nitric oxide levels and increase in ROS production are prevented by exercise.

Figure 3.

Aortic nitric oxide (NO) production was tested using a fluorescent DAF probe and normalized by aortic rings pre-treated with L-NAME to determine the background fluorescence. Relative NO production was tested with and without tPVAT treatment in LZR (A) and OZR (B) (n=7–8). Superoxide dismutase (SOD) activity was tested in LZR (C&D) and OZR (E&F) aortic rings (n=6–8) and tPVAT (n=3–6). Finally, relative ROS production measured by coumarin boronic acid was tested in donor aortic lysates following the treatment with tPVAT (n=4–8) and in tPVAT lysates (n=3) of LZR (G&H) and OZR (I&J), *denotes significant differences within treatments i.e., aortic ring with tPVAT compared to aortic ring with tPVAT or aortic ring only compared to aortic ring only. #denote significant effect of tPVAT, i.e., comparing aortic ring only to aortic ring with tPVAT. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, #p<0.05, ##p<0.01, ####p<0.0001 as measured by one-way ANOVA with holm-sidak post-hoc analysis.