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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Immunobiology. 2021 Apr 6;226(3):152089. doi: 10.1016/j.imbio.2021.152089

Figure 3. FL RAG2-expressing pre-B cells show increased pre-B cell proliferation after 24 hrs post-IR.

Figure 3.

(A) Cell proliferation analysis using flow cytometry. Proliferation was measured by the decrease in Cell Tracker Orange (CMTMR) label due to cell division. Samples were treated with IR, labeled with CMTMR, and then returned to culture for the indicated times before flow cytometry. In (B) is the difference in peak mean fluorescence intensity between 24 and 48 hrs following IR for each sample. The data are a representative example of 3 separate trials.