Figure 1.

Expression of EGFP-p62 causes TDP-43^WT cytoplasmic mislocalisation in a UBA domain dependent manner. Cells co-expressing TDP-43^WT and either EGFP or EGFP-p62 (WT or ΔUBA) were fixed and stained with Hoechst then visualized by confocal microscopy. Arrows show TDP-43 forming a shell around wild type p62 (inset). Three independent experiments were performed. Cells expressing both TDP-43-tdTomato and EGFP construct were imaged from random fields of view with a minimum of 10 cells imaged per experiment. TDP-43-tdTomato co-localisation with the nucleus was determined by calculating the Pearson’s co-efficient with ImageJ. Post-hoc ANOVA Bonferroni test was performed in SPSS.