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. 2021 Jun 1;11:11474. doi: 10.1038/s41598-021-90822-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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p62 overexpression leads to loss of TDP-43-mediated RNA regulation. Effects of p62 overexpression on alternative splicing of selected genes, showing RT-PCRs across Stag2 (A), Poldip3 (B) and Madd (C) transcripts following transfection with p62 overexpression vector (p62) or the empty vector control (EV) and incubated for 1, 3 and 5 days. An RT-PCR across Smn transcripts were used as a loading control. A 100 bp ladder was loaded on either side of the gel and a no template control (NTC) was included in the final lane. Full-length (FL) and exon skipped (Δ) transcripts are annotated and the percentage of specific transcripts are detailed below each lane.