Figure 11.

TDP-43 aggregation and cleavage require the PB1 and UBA domains, and the nuclear export signal of p62. NSC-34 cells were transfected with TDP-43-tdTomato and EGFP-p62 wild type [WT] or mutants as indicated. (A) Schematic diagram of p62 domain structure and deletion mutants: 1–122 (PB1 domain only), 124–440 (lacks the PB1 domain), 1–385 (lacks the UBA domain), Δ122–386 (PB1 and UBA domains only), Δ303–320 (lacks the nuclear export sequence) and K7A/D69A (self-dimerization mutant). (B) Soluble and insoluble fractions were prepared and separated by SDS-PAGE and transferred to nitrocellulose membranes. Western blot analyses were performed as indicated. Image is representative of 3 independent experiments.