Figure 12.

TDP-43 nuclear depletion requires the PB1 and UBA domains of p62, but not the nuclear export signal or self-dimerisation via the PB1 domain. (A) NSC-34 cells were transfected with TDP-43-tdTomato and EGFP empty vector (EGFP-EV) or EGFP-p62 (WT or deletion constructions as indicated). Cells were fixed and stained with the nuclear stain Hoechst. Images are representative of 3 independent experiments. Minimum number of cells analysed per construct = 32. (B) TDP-43-tdTomato co-localisation with the nucleus was determined by calculating the Pearson’s co-efficient with ImageJ. Statistical analyses were performed using SPSS, with Bonferroni and Dunnet T3, p = 0.05. #compared with EGFP-EV, *compared with EGFP-p62 WT.