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. 2021 Jun 1;11:11474. doi: 10.1038/s41598-021-90822-2

Figure 4.

Figure 4

p62 expression induces TDP-43 cleavage to a 35-kDa fragment. Cells were transfected with constant TDP-43-tdTomato with increasing amounts of EGFP-p62 (0, 0.25, 0.5, 1, 1.5 or 2 μg) and decreasing amounts of EGFP (2, 1.75, 1.5, 1, 0.5 or 0 μg) to maintain consistent DNA amounts across transfection. Cells were then lysed in Triton-X buffer (soluble) and Triton-X insoluble pellets were solubilized with SDS (insoluble). Western blots for GFP, RFP and α-tubulin were performed as indicated. Image is representative of 3 independent experiments.