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p62-mediated neuronal death is independent of TDP-43 expression. (A) Cells were transfected with a PMO to knock-down TDP-43 (TDP-43 KD) or a standard control (GTC). The following day cells were transfected with EGFP-p62 and 48 h later trypsinised, incubated with Sytox-Red and then assessed for cell viability by flow cytometry. (B) Cells were harvested at 3 and 5 days post transfection with PMOs and western blots performed as indicated.
