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. 2021 Jun 2;12(6):566. doi: 10.1038/s41419-021-03839-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Schematic representation of the experimental workflow. This figure was made with Servier Medical Art templates, which is licensed under a Creative Commons Attribution 3.0 Unported License: https://smart.servier.com. B Preservation of cell viability after in vitro labelling of Luc⁺MSC with VT680. Note than VT680 labelling produced minimal changes in cell viability of Luc⁺MSC and that the majority of cells were not affected by the second labelling. C The time-course images of BLI and FLI signals produced by double-labelled MSC after subcutaneous transplantation in a NOD mouse. The quantitative data from five mice is given on the right side. Data are plotted as mean ± SEM. Note the high increase in the BLI signal at day 1. However, due to the large variability in the animal responses, the increase is not statistically significant (one-way ANOVA followed by Tukey’s test).