Table 1.

Summary of drug-screening results.

	1 s timeframe	30 s timeframe
Number of starting drugs in the library	84	84
Number of drugs not toxic	68	68
Number of drugs which induced consistent light-off scotopic VMR in both replicates	5	4
Number of drugs which induced consistent light-off scotopic VMR in both replicates, and significantly different from DMSO-treated controls (p value < 0.05)	0	1

The 84 drugs in the ENZO Redox library were each applied to the Q344X larvae at 10 μM (N = 24 larvae) in two independent replicates. Of these 84 drugs, 16 were toxic. The two replicates were then compared to each other with a High-Dimensional Nonparametric Multivariate Test to determine similarity in either 1-s or 30-s timeframe. The drugs that induced consistent light-off scotopic VMR were compared to DMSO-treated Q344X controls to determine if they caused a significant change in behavior (High-Dimensional Nonparametric Multivariate Test, p value < 0.05). In the 1-s timeframe, no drugs met all criteria, but in the 30-s timeframe, one drug (carvedilol) was both consistent in the two replicates and caused a significant change in light-off scotopic VMR compared to controls.