Figure 6.

Hyperspectral images of H&E stained vehicle control and Al₂O₃ NP dosed A-B) liver, C-D) spleen, E-F) lymph node, and G-H) kidney. Mapped areas are highlighted in the HSI images and indicated by circles. A magnification outlined with a white square is inserted for each of the Al₂O₃ NP tissues to show the cellular location of NPs and the mapped areas are shown with white arrows. A, C, D, E, G, H) are male tissues and B, F) are female tissues, no visible differences were observed between male and female tissues. The presence of Al₂O₃ NP was scored in the tissues: 0 = no NP signals detected; 1 = 1 – 10 NP signals detected; 2 = 11 – 25 NP signals detected; 3 = 26 – 50 NP signals detected; 4 = 51 – 100 NP signals detected; 5 = >100 NP signals detected. The score for Al₂O₃ NP was highest in the liver compare to spleen, lymph nodes, and kidney, showing mean ± standard deviation