Figure 3.

The plasma proteome reflects platelet and immune activation

Plasma proteins were quantitated by a dual-antibody proximity extension assay (Olink).

(A) Data from all 10 donors were combined for analysis using a generalized linear mixed effects model (GLMEM) and only proteins exhibiting significant change are shown. Proteins (columns) are organized by unsupervised hierarchical clustering (dendrogram at top), shown by time point (row groupings) post blood draw and donor (rows), and colored by normalized change from the mean for each time point relative to the 2-hour time point.

(B and C) All proteins in Experiment 1 (B) and Experiment 2 (C) were plotted for fold change and adjusted p value (Benjamini-Hochberg FDR), as calculated using a GLMEM model, and colored by time point.

(D) A selection of proteins showing the greatest fold change in combined analysis were plotted by time point, and shaded regions indicate the 95% confidence interval. Where both experiments identified a significant fold change for a given protein but those fold changes differed between experiments according to the GLMEM model, two lines are shown (dashed for Experiment 1, dotted for Experiment 2); otherwise, a single line (solid) representing data from both experiments is shown.

See also Table S2.