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. 2021 Jun 1;11:11466. doi: 10.1038/s41598-021-90776-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In-vivo imaging of ocular tissue using PARS. Vasculature of the iris imaged from ~ 2.5 mm × 2.5 mm area (a) Vasculature around the iris imaged with PARS (b) In-vivo images of melanin content in RPE and choroid layers (c) Images acquired using multiwavelength PARS system at 532 nm (d) 558 nm (e) and the corresponding SO₂ map (e).