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. 2021 Jun 1;12:3184. doi: 10.1038/s41467-021-23378-4

Fig. 4. KCTD19 was identified as a ZFP541-interacting factor in the testis.

Fig. 4

a Immunoblot showing the immunoprecipitates of ZFP541 and KCTD19 from chromatin extracts of WT mouse testes. The data were acquired from two separate experiments. b The tissue-specific expression pattern of Kctd19 was examined using RT-PCR. Testis RNA was obtained from embryonic day 18 (E18), 3-weeks old (3w), and 8-weeks old (8w) male mice. Ovary RNA was obtained from adult 8-weeks old (8w) female mice. RT− indicates control PCR without reverse transcription. The data were acquired from two separate experiments. c Chromosome spreads of WT spermatocytes and spermatids were immunostained as indicated. Dip: Diplotene, rSt: round spermatid, eSt: elongated spermatid. Independent germ cells (at least N = 3) for each stage were examined in a single experiment. Scale bar: 5 μm. d–f Seminiferous tubule sections in WT testis (8-weeks old) were immunostained as indicated. Z: zygotene, Pa: pachytene, ePa: early pachytene, Dip: diplotene, rS: round spermatid, eS: elongated spermatid. Boundaries of the seminiferous tubules are indicated by white dashed lines. Roman numbers indicate the seminiferous tubule stages. Biologically independent mice (N = 2) were examined in two separate experiments. Scale bars: 25 μm for (d), 15 μm for (e), and (f).