Figure 2.
SAA1 promotes HSC recruitment in vitro and in vivo
(A) Representative IHC immunostaining images showing staining of α-SMA-positive cells in SAA1-siRNA2, Neg-siRNA, and control samples obtained from CCl4 and CI injury models. Bar graph represents quantification of IHC images per 5 fields.
(B and C) Representative confocal immunofluorescence images showing co-localization of SAA1 and HSCs in SAA1-siRNA2, Neg-siRNA, and control in CCl4 and CI injury models.
(D and E) Transwell migration assay representing co-culture of primary mouse hepatocytes (PMHep) isolated from injured and healthy mouse with JS1 cells (D) and HepG2 cells (SAA1 overexpressing and GFP expressing) with LX-2 (E). The bar graph represents quantification of relative number of migrated cells.
(F and G) Agarose spot (F) and Transwell (G) migration assays showing migration of LX-2 cells toward rhSAA1 at indicated time intervals. The bar graph represents quantification of relative number of migrated cells.
(Scale bar = A, B, and C/200; G/100 μm: Inset/50 μm). Where applicable, data represent mean ± SEM ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and (n = 3). iPMHep represents injured primary mouse hepatocytes, hPMHep represents healthy primary mouse hepatocytes, HepG2 (SAA1) represents HepG2 cells overexpressing SAA1, and HepG2 (GFP) represents HepG2 expressing GFP.