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. 2021 May 18;12:672143. doi: 10.3389/fimmu.2021.672143

Figure 4.

Figure 4

TLR9 and CD40-targeting vaccination depends on pDC to promote human B cell maturation and IgG induction in humanized mice. Humanized mice were vaccinated as in Figure1 except one group of the mice were treated with pDC depletion Ab before vaccination (A) IFN-α in the plasma 24 hours after vaccination. (B) Expression of CD10 on B cells from spleen at termination. (C) Total IgG level in serum was detected by ELISA. (D) Antigen specific IgG level in the plasma was detected by ELISA. Each dot represents one individual mouse, bars indicate mean. Shown are representative data (PBS, n=3; Vac, n=3; CpG-B+Vac, n=4; CpG+Vac+15B, n=3, for A and B) or combined data (PBS, n=6; Vac, n=3; CpG-B+Vac, n=9; CpG+Vac+15B, n=7, for C and D) of two independent experiments with mean values. *P < 0.05, by one-way analysis of variance (ANOVA) and Tukey’s multiple comparisons test. (E, F) Correlation analysis between the IFN-α levels in plasma and total IgG (E) and specific IgG (F) levels in plasma (Spearman rank correlation test). r, correlation coefficient.