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. 2021 May 18;11:677089. doi: 10.3389/fcimb.2021.677089

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Copyright © 2021 Ji, Wang, Xu, Zhou, Chen, Zhu and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multiplex PCR assay for the identification of four species belonging to ECC. Visualization of the PCR products on an agarose (2.0%) gel for four type strains. Lane M, DNA ladder; Lan1, negative control; Lane 2, the PCR product derived from primers EC-F/R and E. cloacae ssp. cloacae ATCC 13047; lane 3, the PCR product derived from primers EH-F/R and E. hormaechei ssp. hormaechei ATCC 49162; lane 4, the PCR product derived from primers ER-F/R and E. roggenkampii DSM 16690; lane 5, the PCR product derived from primers EK-F/R and E. kobei DSM 13645; Lane 6, the multiplex PCR product.