TABLE 8.
Quantitative trait loci for grain Fe (GFe) and Zn (GZn) content in RIL over three locations.
| QTL name | LG | Position (cM) | Left marker | Right marker | Intermarker distance (cM) | LOD | LOD | LOD | PVE | PVE | PVE | Add | AbyE_01 | AbyE_02 | AbyE_03 | Left CI | RightCI |
| (A) | (A by E) | (A) | (A by E) | ||||||||||||||
| QGFe.E1.2014–2016_Q1 (QFe2.1) | 2 | 138.9 | Ppmsb 001 | Ppmsb 002 | 32.8 | 23.16 | 23.15 | 0 | 18.34 | 18.27 | 0.07 | 3.46 | 0.26 | –0.26 | 0 | 135.9 | 141.9 |
| QGFe.E1.2014–2016_Q2 (QFe3.1) | 3 | 121 | IPES0142 | IPES 0180 | 2.1 | 19.11 | 19.1 | 0.01 | 14.99 | 14.97 | 0.01 | 3.13 | 0.08 | –0.13 | 0.06 | 110 | 124 |
| QGFe.E1.2014–2016_Q3 (QFe5.1) | 5 | 21.3 | IPES 0157 | IPES 0093 | 1.4 | 24.92 | 24.69 | 0.23 | 19.66 | 19.58 | 0.09 | 3.58 | –0.16 | 0.34 | −0.18 | 19.3 | 30.3 |
| QGFe.E1.2014–2016_Q4 (QFe 7.1) | 7 | 24.9 | IPES 0206 | IPES 0015 | 12.3 | 20.06 | 20.03 | 0.02 | 15.66 | 15.61 | 0.05 | 3.21 | 0.25 | –0.1 | −0.14 | 21.9 | 27.9 |
| QGZn.E1.2014–2016_Q1 (QZn 2.1) | 2 | 138.9 | Ppmsb 001 | Ppmsb 002 | 32.8 | 21.39 | 20.85 | 0.54 | 25.95 | 24.72 | 1.23 | 4.51 | 1.42 | –0.83 | −0.58 | 133.9 | 141.9 |
| QGZn.E1.2014–2016_Q2 (QZn 3.1) | 3 | 101 | IPES 0166 | Xpsmp 2249 | 14.0 | 5.86 | 1.74 | 4.11 | 6.2 | 1.92 | 4.28 | 1.26 | –1.5 | 2.65 | −1.15 | 98 | 102 |
| QGZn.E1.2014–2016_Q3 (QZn 3.2) | 3 | 121 | IPES 0142 | IPES 0180 | 2.1 | 11.97 | 8.44 | 3.53 | 14.39 | 9.53 | 4.86 | 2.8 | 1.36 | –2.83 | 1.47 | 108 | 124 |
| QGFe.E2.2014–2016_Q1(QFe2.1) | 2 | 138.9 | Ppmsb 001 | Ppmsb 002 | 32.8 | 23.61 | 23.57 | 0.05 | 17.98 | 17.95 | 0.02 | 4.22 | –0.09 | 0.2 | −0.11 | 133.9 | 141.9 |
| QGFe.E2.2014–2016_Q2 (QFe3.1) | 3 | 121 | IPES 0142 | IPES 0180 | 2.1 | 21.32 | 21.26 | 0.06 | 16.26 | 16.25 | 0.01 | 4.02 | –0.03 | 0.13 | −0.1 | 114 | 124 |
| QGFe.E2.2014–2016_Q3 (QFe5.1) | 5 | 21.3 | IPES 0157 | IPES 0093 | 1.4 | 24.8 | 24.61 | 0.19 | 18.83 | 18.77 | 0.06 | 4.32 | 0.06 | –0.32 | 0.26 | 19.3 | 32.3 |
| QGFe.E2.2014–2016_Q4 (QFe 7.1) | 7 | 24.9 | IPES 0206 | IPES 0015 | 12.3 | 22.02 | 21.97 | 0.05 | 16.61 | 16.61 | 0 | 4.08 | –0.08 | 0.03 | 0.06 | 21.9 | 27.9 |
| QGZn.E2.2014–2016_Q1(QZn 2.1) | 2 | 138.9 | Ppmsb 001 | Ppmsb 002 | 32.8 | 24.89 | 24.58 | 0.31 | 21.88 | 21.77 | 0.11 | 5.05 | –0.46 | 0.39 | 0.07 | 135.9 | 139.9 |
| QGZn.E2.2014–2016_Q2 (QZn 3.1) | 3 | 121 | IPES 0142 | IPES 0180 | 2.1 | 18.86 | 18.39 | 0.46 | 16.07 | 16.05 | 0.02 | 4.34 | –0.07 | 0.22 | −0.14 | 106 | 124 |
| QGFe.E3.2014–2016_Q1(QFe 1.1) | 1 | 452.4 | IPES 0101 | IPES 0139 | 29.1 | 3.94 | 3.93 | 0.01 | 2.92 | 2.91 | 0 | –1.56 | –0.06 | 0.09 | −0.03 | 435.4 | 461.4 |
| QGFe.E3.2014–2016_Q2 (QFe2.1) | 2 | 138.9 | Ppmsb 001 | Ppmsb 002 | 32.8 | 20.63 | 20.61 | 0.01 | 15.98 | 15.98 | 0.01 | 3.54 | 0 | –0.08 | 0.08 | 133.9 | 141.9 |
| QGFe.E3.2014–2016_Q3 (QFe2.2) | 2 | 644.9 | IPES 0027 | IPES 0236 | 23.7 | 3.93 | 3.92 | 0.01 | 2.85 | 2.85 | 0.01 | –1.55 | –0.06 | 0.09 | −0.03 | 633.9 | 657.9 |
| QGFe.E3.2014–2016_Q4 (QFe 3.1) | 3 | 105 | IPES 0142 | IPES 0180 | 2.1 | 19.26 | 19.23 | 0.03 | 14.8 | 14.8 | 0 | 3.41 | –0.02 | 0.01 | 0.01 | 104 | 112 |
| QGFe.E3.2014–2016_Q5 (QFe 5.1) | 5 | 21.3 | IPES 0157 | IPES 0093 | 1.4 | 21.76 | 21.73 | 0.02 | 16.85 | 16.85 | 0 | 3.64 | –0.01 | –0.03 | 0.03 | 19.3 | 34.3 |
| QGFe.E3.2014–2016_Q6 (QFe 7.1) | 7 | 24.9 | IPES 0206 | IPES 0015 | 12.3 | 18.97 | 18.96 | 0.01 | 14.56 | 14.55 | 0.01 | 3.39 | 0.08 | –0.13 | 0.05 | 21.9 | 27.9 |
| QGZn.E3.2014–2016_Q1 (QZn 2.1) | 2 | 140.9 | Ppmsb 002 | IPES 0181 | 45.1 | 11.94 | 10.95 | 0.99 | 11.18 | 10.23 | 0.95 | 3.98 | 0.68 | –1.7 | 1.01 | 139.9 | 143.9 |
| QGZn.E3.2014–2016_Q2 (QZn 3.1) | 3 | 105 | IPES 0142 | IPES 0180 | 2.1 | 25.42 | 24.39 | 1.02 | 22.26 | 21.08 | 1.19 | 5.71 | –1 | 1.92 | −0.92 | 104 | 122 |
| QGZn.E3.2014–2016_Q3 (QZn 6.1) | 6 | 478 | IPES 0141 | IPES 0151 | 27.4 | 3.71 | 3.55 | 0.16 | 2.93 | 2.87 | 0.06 | –2.19 | –0.32 | –0.12 | 0.45 | 471 | 485 |
LOD, overall LOD score of the QTL; LOD (A), score of the main additive effect; LOD (AbyE), score of the Genotype × Year interaction; PVE, overall proportion of phenotypic variance explained by the QTL in percentage; PVE (A), proportion of the phenotypic variance explained by additive effect at the current scanning position; PVE (AbyE), eProportion of the phenotypic variance explained by the QTL due to Genotype × Year interaction; Add, main additive effect; AbyE_01, AbyE_02, and AbyE_03, estimated additive effect of QTL at the current scanning position for each environment (Delhi, Dharwad, and Jodhpur); LeftCI and Right CI, confidence interval calculated by one-LOD drop from the estimated QTL position.