Figure 6.

RPA promotes BLM-mediated G4 unfolding into the durative linearized state

(A) RPA was incubated with the substrate 3G4L3S first, and then BLM and ATP were introduced in the reaction buffer containing 50 mM KCl and 5 mM MgCl₂.

(B) The FRET distributions of 3G4L3S in different concentrations of RPA and after the addition of BLM to the RPA-bound 3G4L3S.

(C) Representative traces after the addition of BLM and ATP to the RPA-bound 3G4L3S.

(D) RPA and BLM were added simultaneously to the 3G4L3S.

(E) The fractions of different types of traces at different concentrations of RPA and BLM with 25 μM ATP.

(F) Representative traces of 3G4L3S in the mixture of 20 nM RPA, 40 nM BLM, and 25 μM ATP.