Figure 7.

RPA assists BLM to overcome the G4 structure and continue to unwind the downstream duplex DNA

(A) The schematic diagram of the substrate. The ssDNA tail and duplex stem are 14-nt and 27-bp, respectively. There is a 4-nt linker between G4 and duplex.

(B) The remaining fractions of duplexes on coverslip before and 4 min after the addition of 100 nM RPA, 60 nM BLM, and 0.5 mM ATP and the mixture of 100 nM RPA, 60 nM BLM, and 0.5 mM ATP. The error bars were obtained from at least three repetitive experiments. Data are presented as mean ± SEM.

(C) The FRET distributions of DNA molecules on coverslips before and after the addition of proteins.

(D) Selected trace after the addition of 100 nM RPA.

(E and F) Selected traces after the addition of 100 nM RPA, 60 nM BLM, and 0.5 mM ATP.