REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli strain BL21 (DE3)	NEB	Cat#C2527H
E. coli strain Rosetta (DE3)	Boule and Zakian, 2007	N/A
Chemicals, peptides, and recombinant proteins
D-glucose	Sigma-Aldrich	Cat#G8270-100g
glucose oxidase	Sigma-Aldrich	Cat#G2133-50KU
Catalase	Sigma-Aldrich	Cat#C9322-5g
Trolox	Sigma-Aldrich	Cat#238813-1g
Streptavidin	Invitrogen	Cat#43-4301
mPEG-SC	Laysan bio	Cat#mPEG-SC-5000
Biotin-PEG-SC	Laysan bio	Cat#Biotin-mPEG-SC-5000
Oligonucleotides
GGTTGGTGTGGTTGG; see Table S1 for sequences used in smFRET, CD, and FRET-melting assay.	This paper	N/A
GGGTGGGTGGGTGGG; see Table S1	This paper	N/A
GGGTGGGTAGGGTGGG; see Table S1	This paper	N/A
GGGTTGGGTTGGGTTGGG; see Table S1	This paper	N/A
GGGTTAGGGTTAGGGTTAGGG; see Table S1	This paper	N/A
GGGTTTTGGGTTTTGGGTTTTGGG; see Table S1	This paper	N/A
GGGTTTTTGGGTTTTTGGGTTTTTGGG; see Table S1	This paper	N/A
GGGGTTAGGGGTTAGGGGTTAGGGG; see Table S1	This paper	N/A
GGGGTGTGGGGACAGGGGTGTGGGG; see Table S1	This paper	N/A
GGGGGTTAGGGGGTTAGGGGGTTAGGGGG; see Table S1	This paper	N/A
Recombinant DNA
Plasmid: p11d-tRPA	Henricksen et al., 1994	N/A
Plasmid: PET-15b-SUMO	Wu et al., 2015	N/A
Plasmid: PET-28a	Boule and Zakian, 2007	N/A
Software and algorithms
SmCamera	Lee at al., 2019	http://ha.med.jhmi.edu/resources/#1464200861600-0fad9996-bfd4
Matlab2014	MathWorks	https://www.mathworks.com/
Origin 2019	OriginLab	https://www.originlab.com/