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. 2021 May 14;24(5):102488. doi: 10.1016/j.isci.2021.102488

Figure 7.

Figure 7

Systemic administration of monovalent FSTL3-Fc increases muscle mass in healthy mice

(A) Body weight of mice injected with mono-FSTL3-Fc or control Fc before and after the start of the injection. Data are means ± SD from n = 7 (control Fc and 10 mg/kg mono-FSTL3-Fc) or n = 6 (3 mg/kg and 30 mg/kg mono-FSTL3-Fc) independent experiments. Differences between the conditions were analyzed by ANOVA with Tukey-Kramer post hoc test for multiple comparison; n.s., not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

(B) Representative macroscopic images of hindlimb muscles and QF and GC muscles excised from mice with i.p. injection of mono-FSTL3-Fc or control Fc (10 mg/kg). Scale bar: 1 cm.

(C) Normalized weight of muscles excised from mice with i.p. of mono-FSTL3-Fc or control Fc at the indicated protein concentration. Muscle weight was normalized to the body weight. Data are means ± SD from n = 7 (control Fc and 10 mg/kg mono-FSTL3-Fc) or n = 6 (3 mg/kg and 30 mg/kg mono-FSTL3-Fc) independent experiments. Differences between the conditions were analyzed by ANOVA with Tukey-Kramer post hoc test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

(D) Representative cross-sectional images of myofibers in the QF excised from mice with local administration of mono-FSTL3-Fc or control Fc (IHC for laminin). Scale bar: 100 μm.

(E) Quantification and distribution of muscle fiber cross-sectional area of (D). The differences in muscle fiber cross-sectional area were analyzed using Wilcoxon rank-sum test; ∗∗∗∗p < 0.0001.

(F) Normalized weight of tissue of mice after a 2-week-treatment. Tissue weight was normalized to the body weight. Data are means ± SD from n = 7 (control Fc and 10 mg/kg mono-FSTL3-Fc) or n = 6 (3 mg/kg and 30 mg/kg mono-FSTL3-Fc) independent experiments. Differences between the conditions were analyzed by ANOVA with Tukey-Kramer post hoc test; n.s., not significant.

(G) The blood glucose concentration of mice after a 2-week-treatment. Mice were fasted for 2.5 hr. Data are means ± SD from n = 7 (control Fc and 10 mg/kg mono-FSTL3-Fc) or n = 6 (3 mg/kg and 30 mg/kg mono-FSTL3-Fc) independent experiments. Differences between the conditions were analyzed by ANOVA with Tukey-Kramer post hoc test; n.s., not significant.