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. 2021 May 19;9:656849. doi: 10.3389/fcell.2021.656849

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Copyright © 2021 Sokpor, Xie, Nguyen and Tuoc.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schema showing application of clustered regularly interspaced short palindromic repeats (CRISPR)–dCas13 for N⁶-methyladenosine (m⁶A) editing. The m⁶A editing system is made by fusing deactivated Cas13b (dCas13b) with a guide RNA (gRNA) that can specifically target abnormally methylated messenger RNA (mRNA) (pathogenic mRNA) and coupling of CRISPR–dCas13 to an m⁶A factor to effect desired changes in m⁶A modification. Depending on the effector used, it is possible to induce m⁶A deposition, removal, or recognition (binding/reading), leading to the induction of degradation, translation enhancement, or increased stability of the target mRNA.