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. 2021 May 19;9:669285. doi: 10.3389/fcell.2021.669285

FIGURE 6.

FIGURE 6

Down-regulation of DCBLD2 can suppress the EMT signal. (A) Bioinformatics prediction of the signal pathways affected by DCBLD2 in GSCALite Database. (B,C) The effect of DCBLD2 on the mRNA (B) and protein (C) level of the transcription factors ZEB1, ZEB2 and SNAIL upstream of EMT. Forty-eight hours after transfection of siRNA into HCT116 cells, RNA and protein were extracted for q-PCR and Western Blot experiments (***p < 0.001; n = 3, Mann–Whitney test). (D) Forty-eight hours after transfection of siRNA into HCT116 cells, changes in the protein level of N-cadherin, E-cadherin, Vimentin and α-SMA, the key protein factors of EMT after 5-FU or DMSO (5 μM) treatment detected by Western Blot experiment (*p < 0.05, **p < 0.01, ***p < 0.001; n = 3, Mann–Whitney test).