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. 2021 May 19;9:669285. doi: 10.3389/fcell.2021.669285

FIGURE 7.

FIGURE 7

DCBLD2 can combine with ITGB1, the key signal factor of focal adhesion pathway. (A,B) Molecular Function Analysis (A) and KEGG Enrichment Analysis (B) of proteins screened out by TAP-MS experiment. (C) Enrichment analysis of DCBLD2 downstream genes obtained from analysis of TCGA data of patients with colorectal cancer. (D) Correlation between the expression of DCBLD2 and ITGB1 at the mRNA level obtained from analysis of TCGA data of patients with colorectal cancer (p < 0.05, n = 379). (E,F) Study the effect of DCBLD2 on the mRNA and protein expression levels of ITGB1 by q-PCR (E) and Western blot experiments (F) (***p < 0.001; n = 3, Mann–Whitney test). (G) Immunofluorescence assay to detect the localization of DCBLD2 and ITGB1 in HCT116. Scale bars: 5 μm. (H) Co-IP of DCBLD2 and ITGB1. HCT116 cells were immunoprecipitated using DCBLD2 or ITGB1 Abs and immunoblotted with ITGB1 or DCBLD2 Abs, respectively. (I) A graphical abstract of this study.