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. 2021 May 19;12:672737. doi: 10.3389/fimmu.2021.672737

Figure 5.

Figure 5

Abacavir diversifies the HLA-B*57:01 peptide repertoire by enhancing the loading of specific peptides. Conditional ligand loaded HLA-B*57:01fos molecules were UV exposed before the binding of the indicated peptide (A = KTFK*DVGNLL, B = TSLK*SRVTI, C = LTTK*LTNTNI, D = NTVELRVK*I, E = ATFK*GIVRAI, F = KVFK*LQTSL, G = VTKK*TYEIW, H = ITTK*AISRW, I = RVDPAK*GLFYF) was followed at 25°C in the presence or absence of excess abacavir and/or monomeric or ERp57 C60A conjugated tapasin-jun. Binding of fluorescent peptide is reported in millipolarisation units (mP). Unbound fluorescent peptide is assumed to have an mP level of 50. Data shown are representative of triplicate or greater experiments. The extent that tapasin, abacavir, or the combination of both enhanced the binding of fluorescent peptides to HLA-B*57:01fos molecules in the replicate experiments is presented in Figure 6 . Colour coding is as follows: grey—peptide alone, blue—abacavir, red—tapasin, green—abacavir + tapasin.